Comparison of the effects of clomiphene citrate and HCG on spermatogenesis and testicular tissue structure in adult male rats

Saeb, Farzaneh and Bashtani, Elham (2019) Comparison of the effects of clomiphene citrate and HCG on spermatogenesis and testicular tissue structure in adult male rats. Doctoral thesis, Gonabad University of Medical Sciences.

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Abstract

Objective:The purpose of this study was to investigate the effect of clomiphene citrate and HCG on the structural changes, as well as the evaluation of the expression of CatSper1, CatSper2, LHCGR, and SF1 genes in testicular tissue of rats. Methods and Materials: Forty male Wistar rats were randomly divided into five groups as follows; G1 as the control group that received normal saline, G2 received olive oil, G3 received 100 IU/kg HCG, G4 received 5mg/kg clomiphene citrate, and G5 received 5 mg/kg clomiphene citrate and 100 IU/kg HCG. The duration of the treatment period was 56 days. At the end of the experiment period, blood samples were taken from all groups, and the serum was isolated. The levels of testosterone, LH, and FSH were assessed by the ELISA method. Histomorphometric analysis along with sperm count was performed to evaluate the morphology of spermatozoa and the number of sperm cells, respectively. Histological examinations were also carried out to determine the mean Johnson score, diameter of the seminiferous tubules, as well as the number of spermatogonia, primary spermatocytes, sertoli cells, and spermatid cells. Real-time PCR was implemented to measure the expression of CatSper1, CatSper2, LHCGR, and SF1 genes. Results: The results showed that the concentrations of testosterone, FSH, and LH were decreased in the G4 group whereas these parameters were increased in the G3 group. A comparison of the sperm quality indicated a significant reduction in the quality of sperm cells in the G4 group compared with other groups. The quality of sperm was significantly enhanced in the G3 and G5 groups in comparison with the G1 group. Also, our findings demonstrated that the expression of CatSper1, CatSper2, LHCGR, and SF1 genes were significantly elevated in the G3 group when compared with other experimental groups. Conclusions: According to the obtained results, it seems that clomiphene citrate reduces the process of spermatogenesis and the detrimental impacts of this compound would be neutralized by the administration of HCG. Keywords: Human chorionic gonadotropin; Clomiphene citrate; Spermatogenesis; Testicular tissue; Follicle-stimulating hormone; Luteinizing hormone

Item Type: Thesis (Doctoral)
Subjects: R Medicine > R Medicine (General)
Depositing User: Unnamed user with email eprints@gmu.ac.ir
Date Deposited: 05 Nov 2019 05:42
Last Modified: 05 Nov 2019 05:42
URI: http://eprints.gmu.ac.ir/id/eprint/1285

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