Effects of lipopolysaccharide-loaded PLGA nanoparticles in mice model of asthma by sublingual immunotherapy

Khakzad, Mohammad Reza and Hajavi, Jafar and Sadeghdoust, Mohammadamin and Aligolighasemabadi, Farnaz (2019) Effects of lipopolysaccharide-loaded PLGA nanoparticles in mice model of asthma by sublingual immunotherapy. International Journal of Polymeric Materials and Polymeric Biomaterials. pp. 1-8.

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Abstract

The aim of this study was to encapsulation LPS into PLGA nanoparticles and evaluation of effects by SLIT in asthmatic BALB/c mice. LPS-loaded PLGA NPs were prepared in water-in oil-in water method. Sensitized BALB/c mice to ovalbumin as allergen, LPS-loaded PLGA NPs in different low and high doses were administered sublingually. Serum and splenocytes level of some cytokines, also, BALF and lungs histological analysis was performed. SLIT by LPS-PLGA 200nm treatment showed significantly increased the cytokine. The study of BALF and histological examination indicates the reduction of cells and eosinophilic infiltration. The LPS-PLGA NPs could effectively shifted Th2 to Th1/Treg. GRAPHICAL ABSTRACT ARTICLE HISTORY Received 19 October 2018 Accepted 17 December 2018 KEYWORDS PLGA-LPS; LPS low dose; LPS high dose; sublingual immunotherapy; asthma

Item Type: Article
Subjects: R Medicine > RZ Other systems of medicine
Depositing User: Unnamed user with email eprints@gmu.ac.ir
Date Deposited: 31 Dec 2019 09:56
Last Modified: 31 Dec 2019 09:56
URI: http://eprints.gmu.ac.ir/id/eprint/1786

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