LC-ESI/LTQOrbitrap/MS/MS and GC–MS profiling of Stachys parviflora L. and evaluation of its biological activities

Shakeri, Abolfazl and D’Urso, Gilda and Taghizadeh, Seyedeh Faezeh and Piacente, Sonia and Norouzi, Saeed and Soheili, Vahid and Asili, Javad and Salarbashi, Davoud (2019) LC-ESI/LTQOrbitrap/MS/MS and GC–MS profiling of Stachys parviflora L. and evaluation of its biological activities. Journal of Pharmaceutical and Biomedical Analysis, 168. 209 - 216.

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Abstract

The use of some Stachys genus as herbal remedies is known and the aerial parts have a pharmaceutical interest, being used in Anatolia and Iran as wild tea. In this study, chemical composition, antimicrobial, antioxidant, and antiproliferative activities of the methanolic extract and essential oil (EO) of Stachys parviflora L. (S. parviflora) were evaluated. Qualitative analysis of metabolites of S. parviflora methanolic extract was studied using liquid chromatography coupled to high resolution mass spectrometry (LC-ESI/LTQOrbitrap/MS), evidencing the presence of phenolic acids and flavonoids derivatives. The EO was analyzed using gas chromatography coupled to mass spectrometry (GC/MS). Eighty-seven compounds were characterized in the EO of S. parviflora, of which α-terpenyl acetate (23.6), β-caryophyllene (16.8), bicyclogermacrene (9.3), spathulenol (4.9) and α-pinene (4.2) were found to be the major components. The highest antimicrobial effect of EO was found to S. aureus and B. cereus (MIC = 0.01 μg/ml), while the highest activity of extract was against B. cereus (MIC = 125 μg/ml). The methanolic extract exhibited strong antioxidant activity in DPPH (IC50 = 76.87 μg/ml) and β-carotene/linoleic acid assay (BCB, IC50 = 188.47 μg/ml) methods. Furthermore, in vitro cytotoxicity evaluation against three cell lines namely human ovarian carcinoma (A2780), human colon carcinoma (HCT), and mouse melanoma cell line (B16F10), showed an anti-proliferative activity of the EO ranging from IC50 value 30.95 μg/ml to 16.55 μg/ml. The results from this study have demonstrated the promising cytotoxic, antibacterial, and antifungal properties of S. parviflora, which could have wide potential applications in food and pharmaceutical industries.

Item Type: Article
Uncontrolled Keywords: , LC–MS, Essential oil, Cytotoxicity, Antimicrobials, Antioxidant
Subjects: R Medicine > RZ Other systems of medicine
Depositing User: Unnamed user with email eprints@gmu.ac.ir
Date Deposited: 14 Apr 2019 07:21
Last Modified: 14 Apr 2019 07:21
URI: http://eprints.gmu.ac.ir/id/eprint/537

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