Correlation between expression of CatSper1,2 and sperm parameters in the gamma irradiated adult mouse testis.

Mohammadi, Shabnam and Kianmehr, Mojtaba and Mohammadi, Maryam and Fahimian, Zahra and Karimimanesh, Elham and Farazifar, Mostafa and Nakhaei, Zahra and Golamneghad, Nafiseh and Bolourifard, Basir and Gholamin, Mehran and Mansouri, Atena and Mahmoodi M, Reyhaneh Sadat and Soleymanifard, Shokouhozaman and Boroumand-Noughabi, Samaneh and Ghandy, Nasibeh and Delshad, Ali and Mohammadzadeh, Fatemeh and Norasteh, Hojjat and Ghayour-Mobarhan, Majid and Ferns, Gordon Aa (2019) Correlation between expression of CatSper1,2 and sperm parameters in the gamma irradiated adult mouse testis. International journal of radiation biology, 95 (6). pp. 691-696. ISSN 1362-3095

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Abstract

CatSper protein channels are responsible for the entry of Ca2+ into sperm cells. These proteins play an important role in motility and male fertility. So it is important to find out whether or not environmental factors, such as gamma radiation, have an effect on the expression of Catsper genes. In this study, we investigated the effects of gamma radiation on the expression of CatSper1 and CatSper2 genes. Twenty-one male NMRI mice were divided into three groups: a control group without gamma radiation, and two experimental groups; Group 1 treated with 1 Gy of gamma radiation, and Group 2 treated with a higher dose of 2 Gy gamma radiation. Testes were removed from all groups of animals 35 days following irradiation and the testicular tissue, processed and embedded in paraffin blocks for sectioning and histological examination. Sperm samples were also taken from the epididymis for microscopic. Sperm parameters such as sperm count, morphology, motility, and viability rates were analyzed. Expression of CatSper genes was evaluated using Real-time PCR. Data were analyzed using the SPSS software and ANOVA test. Our results showed that after treatment with gamma radiation, testes morphology was changed. Epididymal sperm count, motility, and morphology rates were significantly affected in both experimental groups compared to the control group. The relative expressions of CatSper 1 and 2 genes were significantly reduced in the irradiated mice (1 Gy and 2 Gy) than non-irradiated ones. Gamma radiations not only change testes histology and sperm parameters, but also decrease the expression of CatSper 1 and 2 genes in male mice.

Item Type: Article
Subjects: R Medicine > RZ Other systems of medicine
Depositing User: Unnamed user with email eprints@gmu.ac.ir
Date Deposited: 22 Sep 2019 08:40
Last Modified: 22 Sep 2019 08:40
URI: http://eprints.gmu.ac.ir/id/eprint/592

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