Comparison of DNA damage in blood lymphocytes of indoor pool lifeguard athletes with non-lifeguard athletes

Mottaghi, Mahmoudreza Comparison of DNA damage in blood lymphocytes of indoor pool lifeguard athletes with non-lifeguard athletes. Technical Report. معاونت تحقیقات و فنآوری دانشگاه علوم پزشکی گناباد.

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Abstract

Introduction: Chlorination has been used as a major disinfectant process for public drinking and swimming pool water in many countries. However, it was concern over the disinfection byproducts (DBPs) by the reaction of chlorine with organic material in water and the effect on human health. Trihalomethane is a water-based chlorine-free product that is present in pool water and above the water pool, blood swimmers' bloodstream, swimmers lung ganglia, etc. The purpose of this study was to compare the amount of DNA damage in blood lymphocytes if indoor pool lifeguard athletes with non-lifeguard athletes. Materials and Methods: This was a case-control study. The studied population was Gonabad's lifeguards. Sampling was carried out using simple random method. Sample size was calculated after a pilot study and using the sample size formula based on the difference in mean in two independent groups we chose 30 participants for each group, coming to 60 participants altogether, including the control group. To analyze the collected data, descriptive statistics (mean and standard deviation) and analytical statistics (independent t-test) were used to compare the mean of quantitative variables of case group with control group. Also, Pearson correlation test to examine the relationship between quantitative variables was used. Results: The means of parameters of comet assay, which were used to measure the amount of DNA damage in the two groups, showed a significant difference in the lifeguards (p <0.001). On the other hand, the results of the study showed a higher level of DNA damage than those of lifeguards. For example, tail length in women was 8.97 ± 4.21 and in males, 1.33 ± 4.32, (P = 0.001). Tail DNA (%) in females was 4.18 ± 1.27 and in men, 3.14 ± 0.94, (P = 0.016) and tail moment in women was 0.53 ± 0.68 and in men was 0.14 ± 0.26 (P = 0.010). There was also a significant positive correlation between DNA damage and work experience (P <0.001). Conclusion: DNA damage in lymphocytes of lifeguards is more than that of the control group. It was also more in women than men. So, there is a need for lifeguards who have many hours of exposure to these compounds daily and have higher levels of DNA damage than other people, to reduce their harmful effects. Keywords: DNA damage, lifeguards, indoor pool, chlorine

Item Type: Monograph (Technical Report)
Subjects: R Medicine > RZ Other systems of medicine
Depositing User: Unnamed user with email eprints@gmu.ac.ir
Date Deposited: 13 Oct 2019 04:47
Last Modified: 13 Oct 2019 04:52
URI: http://eprints.gmu.ac.ir/id/eprint/602

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